Part:BBa_K1030008
Zeo-resistant gene + GFPuv
We use a zeocin resistant gene and external zeocin to tune the cell growth rate. The common antibiotic resistant genes are reserved for plasmid backbones. GFPuv is used to monitor the expression of zeocin resistant gene. In addition, zeocin resistant gene allosterically inhibitor the effect of zeocin, makes it easy to be quantified and mathematically modeled.
From the data we can see that when the concentration of zeocin is less than 5000ug/ml, the number of the cells is large enough and increase slowly. But when the concentration of zeocin is more than 5000ug/ml, the cells’ number decrease rapidly. It means that when the the concentration of Zeocin is not too high, the cells can grow well. It shows us that the zeocin does harm to cells but the parts we design can resistant the effect of the zeocin so that cells can grow well. We can use this part and the external zeocin to tune the cell growth rate. It is fun and meaningful.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 644
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